Protein Expression, Cloning & Biotechnology Techniques

[bibliplug last_name=’Baneyx’]

[bibliplug last_name=’Carrio’ year=’2001′]

[bibliplug last_name=’Carrio’ year=’2002′]

[bibliplug last_name=’Cregg’]

[bibliplug last_name=’Geisbrecht’]

General notes

Here are a few pieces of information that may be useful to you:

• You can get petri dishes from Peter Louw; some chemicals as well.
• You get electroporation cuvettes from Blommie
• The chloramphenicol is in the walk-in fridge on the second floor.
• We have our own kanamycin. It is kept at room temp in lab 319.
• Don’t grow bacteria overnight in ampicillin at 37°C, rather do it at 30°C. If the cells grow too fast, the media becomes acidified and this can degrade the ampicillin.