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You are here: Home / Research Projects / Determination of the structure of the plant natiuretic peptide irPNP from Arabidopsis thaliana

Determination of the structure of the plant natiuretic peptide irPNP from Arabidopsis thaliana

Plant natiuretic peptides are peptide hormones involved in water and salt homeostasis in plants (see [cite num=1] or consult Chris Gehring for further details). irPNP from Arabidopsis has a molecular weight of approximately 10kDa, making it suitable for either NMR or X-ray. The structure is currently uncharacterised.

The protein has been recombinantly expressed as a GST fusion and shown to be biologically active, and some optimisation of expression and solubility conditions has been done. The project will involve scale-up of expression and preliminary NMR studies and crystallisation trials. Depending on the success of the former, NMR or X-ray data will be collected and the structure determined in collaboration with either David or Muhammed.

  1. Gehring, C. A., & Irving, H. R. (2003). Natriuretic peptides–a class of heterologous molecules in plants. Int J Biochem Cell Biol, 35(9), 1318-22. PMID: 12798346 [cite num=”1″ return=TRUE]

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