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  • Chromatography
    • Affinity chromatography
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    • Hydrophobic interaction chromatography
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  • Major Infrastructural Components
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  • Protein Preparation Protocols
    • Before you begin
    • Biochemistry and Assays
    • Crystallography
    • Electrophoresis and gel staining
    • Immunoassays and other Immunological Techniques
    • Liquid Handling
    • Other protocols
    • Protein Expression, Cloning & Biotechnology Techniques
    • Protein Purification Techniques
    • Spectrophotometry/UV Detection
  • Software and Equipment Manuals
    • “O”
    • IMAGIC-5
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  • Useful Structural Biology Links
• Structural Biology Coursework Modules
  • [GRD] Laboratory Techniques for Structural Biologists
    • Gel Electrophoresis
      • Gel formulations
      • The BIORAD Mini-PROTEAN 3 cell
      • The BIORAD PROTEAN II xi cell
    • Principles of Chromatography
      • Affinity Chromatography
      • Gel Filtration Chromatography
      • Hydrophobic Interaction Chromatography
      • Ion-Exchange Chromatography
      • Practical Guide to HPLC
    • Measuring Concentrations
      • Precision Volumetric Equipment
    • Measuring pH
    • Preparing Buffer Solutions
    • Determination of concentration
  • [CMP] Computational and Mathematical Primer
    • Introduction to the LINUX operating system
  • [MBBP] Molecular Biology Primer and Biotechnology Program
    • Biophysics of Proteins and Nucleic Acids
  • [PPS] Principles of Protein Structure
  • [PEP] Protein Expression and Purification
  • [SCB] Structural Cell Biology
    • Cells and the Fabric of Life
    • Mitochondria Movies
    • Tomography and Image Interpretation
  • [SBI] Structural Bioinformatics
    • Detailed explanation of protein topology cartoons
    • Protein topology literature review
  • [MM] Molecular Modelling
    • Conformational Analysis
    • Force Fields
    • Molecular Dynamics
    • Normal Mode Analysis
  • [NMR] Protein structure determination using NMR spectroscopy
  • [PC] X-ray crystallography of proteins
    • Electron Crystallography Virtual Course – Erice 2004
    • Introduction to X-ray Crystallography
    • Tutorials in Protein Crystallography
  • [3DEM] Three-dimensional Electron Microscopy
    • 3D Image Reconstruction of Helical Objects
    • 3DEM Online Lectures
  • [APP] Applications of Structural Biology
    • [SDD] Structure-based Drug Design
    • Virology & Vaccines
    • [VCP] Vaccinia Complement Protein
    • [PMM] Proteins as Molecular Machines
• Research Projects
  • Gallery of our solved structures
  • The structure, mechanism and specificity of amidases
  • Mycobacterium tuberculosis MshB
  • Structure determination of ImuC from M. tuberculosis
  • Structure of B. pumilus nitrilase
  • Structure of R. rhodochrous J1 nitrilase
  • 3D reconstruction of chromatin
  • 3D SEM for structural biology
  • B. smithii RAPc8 amidase
  • Crystal Contact Database
  • Human papillomavirus
  • Structure of angiotensin-converting enzyme mutants
  • Structure of glutamine synthetase
  • Virology projects
  • X-ray structure determination of under-glycosylated, truncated angiotensin-converting enzyme (ACE)
  • Determination of the structure of the mitochondrial-associated human protein Endozepine
  • Determination of the structure of the DWNN-containing protein from the protozoon Encephalitozoon cuniculi
  • DNA triplet repeat oligonucleotides
  • Determination of the structure of the plant natiuretic peptide irPNP from Arabidopsis thaliana
  • Determination of the structure of a novel guanylyl cyclase from Arabidopsis thaliana
  • Nitrogen metabolizing enzymes from Bacteroides fragilis (2 projects)
  • The structure of the nitrile hydratase from Bacillus pallidus
  • Nucleosomal location of linker histones
  • Various nitrilase structure projects