Lower the Inner Chamber Assembly into the Mini Tank. Fill the inner chamber with ~ 125 ml of running buffer until the level reaches halfway between the top of the taller and shorter glass plates of the Gel Cassette.

e. Lower the Inner Chamber Assembly into the Mini Tank. Fill the inner chamber with ~ 125 ml of running buffer until the level reaches halfway between the top of the taller and shorter glass plates of the Gel Cassette.

Note: Do not overfill the Inner Chamber Assembly. Excess buffer will cause siphoning of buffer into the lower chamber which can result in buffer loss and interruption of electrophoresis.

Add ~ 200 ml of running buffer to the Mini Tank (Lower Buffer Chamber).