e. Lower the Inner Chamber Assembly into the Mini Tank. Fill the inner chamber with ~ 125 ml of running buffer until the level reaches halfway between the top of the taller and shorter glass plates of the Gel Cassette.
Note: Do not overfill the Inner Chamber Assembly. Excess buffer will cause siphoning of buffer into the lower chamber which can result in buffer loss and interruption of electrophoresis.
Add ~ 200 ml of running buffer to the Mini Tank (Lower Buffer Chamber).