Most of the information you'll ever need is provided on the Amersham website (under Literature » Handbooks). It covers basic principles of protein purification, basic theory of chromatography, how to pack columns, assess packing efficiency, recommended flow rates etc. Other documents on these topics are: One important consideration in gel filtration chromatography is to always […]
Describes methods for dialysis, ultrafiltration, ammonium sulfate precipitation (salting out), ion exchange (followed by desalting chromatography), spin-filters, and trichloroacetic acid/deoxycholate (TCA/DOC)
An Amersham Biosciences document.
By Amersham Biosciences.
Amersham Biosciences Handbook. Affinity chromatography separates proteins on the basis of a reversible interaction between a protein (or group of proteins) and a specific ligand coupled to a chromatography matrix. The technique offers high selectivity, hence high resolution, and usually high capacity for the protein(s) of interest. Purification can be in the order of several thousand-fold and recoveries of active material are generally very high.
A selection of YouTube videos about affinity chromatography - mostly from the manufacturers of the equipment we use in our lab.
HiTrap™ Blue HP is a prepacked ready to use, disposable column for preparative affinity chromatography.
Amersham Application Note: Purification of (His)6-tagged proteins using HiTrap Chelating HP columns charged with different metal ions
Amersham Biosciences: Reactive dye affinity chromatography matrices
Amersham Biosciences Handbook. Gel filtration chromatography seprarates proteins, peptides, and oligonucleotides on the basis of size.
A selection of YouTube videos about gel filtration chromatography - mostly from the manufacturers of the equipment we use in our lab.
A powerpoint presentation by Amersham
Application Note from Amersham BioSciences
Sephacryl™ High Resolution chromatography media is a versatile gel filtration media that offers a wide range of fractionation capabilities. The hydrophilic, rigid matrix of Sephacryl minimizes non-specific adsorption while maximizing recoveries.
Sephacryl™ High Resolution chromatography instructions. Sephacryl™ High Resolution is is one of the Amersham Biosciences range of BioProcess Media, and is a cross-linked copolymer of allyldextran and N,N’-methylene bisacrylamide
Tricorn™ high performance columns.
Polymeric TSK-GEL PW and TSK-GEL PWXL columns are designed for gel filtration of water-soluble organic polymers, proteins, peptides, polysaccharides, oligosaccharides, DNA, and RNA.