Amersham Biosciences Handbook. Affinity chromatography separates proteins on the basis of a reversible interaction between a protein (or group of proteins) and a specific ligand coupled to a chromatography matrix. The technique offers high selectivity, hence high resolution, and usually high capacity for the protein(s) of interest. Purification can be in the order of several thousand-fold and recoveries of active material are generally very high.
A selection of YouTube videos about affinity chromatography - mostly from the manufacturers of the equipment we use in our lab.
HiTrap™ Blue HP is a prepacked ready to use, disposable column for preparative affinity chromatography.
Amersham Application Note: Purification of (His)6-tagged proteins using HiTrap Chelating HP columns charged with different metal ions
Amersham Biosciences: Reactive dye affinity chromatography matrices