Amersham Biosciences Handbook. Ion-exchange chromatography (or ion chromatography) is a process that allows the separation of ions and polar molecules based on their affinity to the ion exchanger. It can be used for almost any kind of charged molecule including large proteins, small nucleotides and amino acids. Chromatofocusing is a protein-separation technique that allows resolution of single proteins and other ampholytes from a complex mixture according to differences in their isoelectric point.
A selection of YouTube videos about ion exchange chromatography - mostly from the manufacturers of the equipment we use in our lab.
ANX Sepharose™ 4 Fast Flow (high sub) ion exchanger is a weak ion exchanger based on highly crossed-linked agarose. It can be used with high flow rates and is well suited for process – scale chromatography. ANX Sepharose 4 Fast Flow (high sub) is part of the Amersham range of separation media called BioProcess™ Media.
CM Sepharose™ Fast Flow, DEAE Sepharose Fast Flow, Q Sepharose Fast Flow and SP Sepharose Fast Flow are ion exchange chromatography media with excellent flow properties and high capacity for biomolecules.
Prepacked, ready-to-use cation and anion exchange HiTrap columns for method scouting, group separations, sample concentration and sample clean-up of charged biomolecules.