Other protocols

Other protocols

  • Enzymes Important for Recombinant DNA Techniques

    A short description of various enzymes useful in recombinant techniques.

  • General notes on basic molecular biology

    Notes on plasmids, and sequencing.

  • PCR (polymerase chain reaction)

    An overview of the polymerase chain reaction.

  • Agarose gel electroporesis

    Agarose gel electrophoresis separates DNA fragments according to their size. Typically, a DNA molecule is digested with restriction enzymes, and the agarose gel electrophoresis is used as a diagnostic tool to visualize the fragments. An electric current is used to move the DNA molecules across an agarose gel, which is a polysaccharide matrix that functions as a sort of sieve to help "catch" the molecules as they are transported by the electric current.

  • Alkaline lysis

    Alkaline lysis is the method of choice for isolating circular plasmid DNA, or even RNA, from bacterial cells. It is probably one of the most generally useful techniques as is a fast, reliable and relatively clean way to obtain DNA from cells. If necessary, DNA from an alkaline lysis prep can be further purified.