[toc]The intention of this module is introduce you to the applications of structural biology by utilizing a practical example, namely, Vaccinia Complement Protein (VCP), and the opportunity to use modern, robotic protein purification equipment.
- Structure of VCP
- Mapping and primary structural similarity.
- Comparison of VCP to the family of complement control protein.
- Demonstration of Over-Expression of VCP.
- NMR structure of VCP.
- X-ray structure of VCP.
- Structure-function relationship of VCP
- Biological functions of VCP.
- Discussion and demonstration of truncation and site specific mutations of VCP.
- Assignment of essential domains for binding to complement components, heparin and suramin.
- Structure-stability relationship of VCP
- Influence of extremes of pH, temperature, freeze thawing, storage and lyophilization on VCP.
- Structural changes and melting curves with adverse physical conditions.
[bibliplug category=”VCP” order_by=”last_name, year, title”]